【佳學(xué)基因檢測】ALDH+ 肺腺癌干細胞中新型多組學(xué)表達圖譜的鑒定和基于圖譜的競爭性內(nèi)源性 RNA 網(wǎng)絡(luò)的元分析

病原微生物基因檢測多少錢要點

體會癌的基因檢測基因解碼如何創(chuàng)新治療認識到《Biomed Res Int》在.?2022 Aug 31;2022:9545609.發(fā)表了一篇題目為《ALDH+ 肺腺癌干細胞中新型多組學(xué)表達圖譜的鑒定和基于圖譜的競爭性內(nèi)源性 RNA 網(wǎng)絡(luò)的元分析》腫瘤靶向藥物治療基因檢測臨床研究文章。該研究由Wei Yang,?Yong Liang,?Yuanyuan Zheng,?Haitao Luo,?Xiaofei Yang,?Furong Li等完成。促進了腫瘤的正確治療與個性化用藥的發(fā)展，進一步強調(diào)了基因信息檢測與分析的重要性。

腫瘤靶向藥物及正確治療臨床研究內(nèi)容關(guān)鍵詞：

腫瘤靶向治療基因檢測臨床應(yīng)用結(jié)果

ALDH+ H1975 肺腺癌干細胞 (LSC) 是在肺腺癌 (LUAD) 中發(fā)現(xiàn)的罕見細胞群。 LSCs 可以自我更新，驅(qū)動腫瘤的發(fā)生、生長、轉(zhuǎn)移和反復(fù)，并且由于其對藥物和化學(xué)療法的內(nèi)在抗性，也是預(yù)后不良的主要原因。因此，LSCs 是 LUAD 治療的一個有希望的靶點。非編碼 RNA (ncRNA)，包括 microRNA (miRNA)、長鏈非編碼 RNA (lncRNA) 和環(huán)狀 RNA (circRNA)，在人類癌癥的發(fā)病機制中發(fā)揮著許多重要的調(diào)節(jié)功能，顯示了全面了解其機制的必要性肺癌的發(fā)生。盡管如此，對許多已知轉(zhuǎn)錄本和信使 RNA (mRNA) 的研究已經(jīng)產(chǎn)生了新的信息。 ncRNA 中的未知生物標志物以及與未知 ncRNA 和 mRNA 的系統(tǒng)和全面的相互關(guān)系可能會為 LUAD 的生物學(xué)提供進一步的見解。在此，我們鑒定了一組新的 ncRNA，包括 miRNA、lncRNA 和 circRNA，并使用嚴格的生物信息學(xué)流程獲得了 LSC 和 ALDH-H1975 LUAD 腫瘤細胞 (LTC) 中 ncRNA 和 mRNA 的差異表達模式。通過對已識別景觀的薈萃分析，構(gòu)建了新的競爭性內(nèi)源性 RNA (ceRNA) 網(wǎng)絡(luò)，以揭示調(diào)節(jié) LSC 和 LTC 標志的潛在分子機制。本研究總結(jié)了新型 ncRNA 以及差異表達的 ncRNA 在 LSC 和 LTC 活性中的基本作用。此外，該研究還為未來識別 LUAD 中的診斷、治療和預(yù)后生物標志物提供了綜合資源。

腫瘤發(fā)生與反復(fù)轉(zhuǎn)移國際數(shù)據(jù)庫描述：

ALDH+ H1975 lung adenocarcinoma stem cells (LSCs) are a rare cell population identified in lung adenocarcinoma (LUAD). LSCs can self-renew, drive tumor initiation, growth, metastasis, and recurrence and are also the predominant cause of poor prognosis due to their intrinsic resistance to drugs and chemotherapy. Consequently, LSCs are a promising target for LUAD therapy. Noncoding RNAs (ncRNAs), including microRNAs (miRNAs), long noncoding RNAs (lncRNAs), and circular RNAs (circRNAs), exert many significant regulatory functions in the pathogenesis of human cancers, showing the necessity for a comprehensive understanding of the mechanisms that underlie lung carcinogenesis. Nonetheless, research on many known transcripts and messenger RNAs (mRNAs) has already generated new information. Unknown biomarkers in ncRNAs and systematic and comprehensive interrelation with unknown ncRNAs and mRNAs may provide further insights into the biology of LUAD. Herein, a set of novel ncRNAs that include miRNAs, lncRNAs, and circRNAs were identified, and differentially expressed patterns of ncRNAs and mRNAs in LSCs and ALDH-H1975 LUAD tumor cells (LTCs) were obtained using stringent bioinformatics pipelines. Through a meta-analysis of the identified landscapes, novel competitive endogenous RNA (ceRNA) networks were constructed to reveal the potential molecular mechanisms that regulate the hallmarks of LSCs and LTCs. This study presents a summary of novel ncRNAs and the fundamental roles of differentially expressed ncRNAs implicated in the activity of LSCs and LTCs. In addition, the study also provides a comprehensive resource for the future identification of diagnostic, therapeutic, and prognostic biomarkers in LUAD.